Coding

Part:BBa_K563031:Design

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pEASY with first part of Tor2 without a original promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2612
    Illegal XbaI site found at 2865
    Illegal SpeI site found at 258
    Illegal PstI site found at 2825
    Illegal PstI site found at 4015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 2825
    Illegal PstI site found at 4015
    Illegal NotI site found at 2846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1775
    Illegal BglII site found at 3802
    Illegal BamHI site found at 252
    Illegal XhoI site found at 2853
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2612
    Illegal XbaI site found at 2865
    Illegal SpeI site found at 258
    Illegal PstI site found at 2825
    Illegal PstI site found at 4015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2612
    Illegal XbaI site found at 2865
    Illegal SpeI site found at 258
    Illegal PstI site found at 2825
    Illegal PstI site found at 4015
    Illegal NgoMIV site found at 3191
    Illegal NgoMIV site found at 4466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5365
    Illegal BsaI.rc site found at 1222
    Illegal SapI.rc site found at 4315
    Illegal SapI.rc site found at 4525


Design Notes

We separate Tor2 into three parts, and the first part is called 01Tor2, including the promoter. For this BioBrick, pEASY is connected with 11Tor2, the first part of Tor2 without a original promoter. pEASY are blunt vector provided by transgene. We link 11Tor2 with this vector for further connection.


Source

11Tor2 is from genomic sequence, and pEASY is from Transgene Company

References